PLZF Monoklonaler Antikörper

PLZF Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2E6F12

Kat-Nr. : 66672-1-Ig

Synonyme

PLZF, ZBTB16, Zinc finger protein 145, Zinc finger protein PLZF, ZNF145

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Galerie der Validierungsdaten

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  WB analysis of Ramos using 66672-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66672-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66672-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66672-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66672-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66672-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66672-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen, Daudi-Zellen, HeLa-Zellen, humanes Hodengewebe, Raji-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

66672-1-Ig bindet in WB, ELISA PLZF und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLZF fusion protein Ag25180
• Vollständiger Name: zinc finger and BTB domain containing 16
• Berechnetes Molekulargewicht: 673 aa, 74 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC026902
• Gene symbol: ZBTB16
• Gene ID (NCBI): 7704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.

Publikationen

Species	Application	Title
mouse	WB	Front Physiol; Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice; Authors - Pan-Pan Lai
mouse	WB	iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
mouse	WB	J Hazard Mater; Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice; Authors - Tianxin Zhao