PLXNB2 Monoklonaler Antikörper

PLXNB2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G9B2

Kat-Nr. : 67265-1-Ig

Synonyme

dJ402G11.3, KIAA0315, MM1, Nbla00445, PLEXB2, plexin B2, PLXNB2

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Galerie der Validierungsdaten

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  WB analysis using 67265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of ATDC-5 using 67265-1-Ig

  ATDC-5 cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 67265-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67265-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67265-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, A549-Zellen, ATDC-5-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67265-1-Ig bindet in WB, IHC, ELISA PLXNB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLXNB2 fusion protein Ag28706
• Vollständiger Name: plexin B2
• Berechnetes Molekulargewicht: 205 kDa
• Beobachtetes Molekulargewicht: 240 kDa
• GenBank-Zugangsnummer: NM_012401
• Gene symbol: PLXNB2
• Gene ID (NCBI): 23654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 20-466aa of human plexin B2 can recognize precursor and alpha subunit of plexin B2.