PLXNB1 Polyklonaler Antikörper

PLXNB1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 23795-1-AP

Synonyme

KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 23795-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23795-1-AP (PLXNB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 23795-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23795-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of SH-SY5Y using 23795-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug PLXNB1 antibody (23795-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

23795-1-AP bindet in WB, IHC, IF, FC, ELISA PLXNB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLXNB1 fusion protein Ag20719
• Vollständiger Name: plexin B1
• Berechnetes Molekulargewicht: 2135 aa, 232 kDa
• Beobachtetes Molekulargewicht: 210 kDa
• GenBank-Zugangsnummer: BC146793
• Gene symbol: PLXNB1
• Gene ID (NCBI): 5364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).

Publikationen

Species	Application	Title
mouse	FC	Cancer Immunol Res; Semaphorin4D Inhibition Improves Response to Immune-Checkpoint Blockade via Attenuation of MDSC Recruitment and Function.; Authors - Paul E Clavijo
human	WB	J Transl Med; SEMA4D/PlexinB1 promotes AML progression via activation of PI3K/Akt signaling.; Authors - Lu Liu
rat	WB	Ann Transl Med; Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.; Authors - Kun Wang
mouse	IF	J Neuroendocrinol; PLXNB1 mutations in the etiology of idiopathic hypogonadotropic hypogonadism.; Authors - Bradley A Welch
human	IHC	Ann Transl Med; Methylation-based epigenetic studies and gene integration analysis of preeclampsia; Authors - Lei Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-04-2021)

calculated size 230 kd. this antibody recognized major bands at 35, 30 kd.

• Applications: Western Blot