PLSCR3 Polyklonaler Antikörper

PLSCR3 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 51061-2-Ig

Synonyme

phospholipid scramblase 3, PL scramblase 3, PLSCR3

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 51061-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 51061-2-Ig(PLSCR3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 51061-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 51061-2-Ig(PLSCR3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 51061-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 51061-2-Ig(PLSCR3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 51061-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 51061-2-Ig(PLSCR3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

51061-2-Ig bindet in WB, IHC, ELISA PLSCR3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: phospholipid scramblase 3
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC093026
• Gene symbol: PLSCR3
• Gene ID (NCBI): 57048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLSCR3, also named as PLS3, belongs to the phospholipid scramblase family. It may mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. PLSCR3 may play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. It is a target of protein kinase C-δ (PKC-δ), resides in mitochondria and plays pivotal roles in regulating apoptotic response. Phosphorylation of PLSCR3 by PKC-δ is involved in the hyperthermia-induced apoptotic signal transduction pathway in Tca8113 cells, and that Hsp27 blocks this pathway to suppress hyperthermia-induced apoptosis. (PMID:20569108)

Publikationen

Species	Application	Title
human	WB	Int J Hyperthermia; Hyperthermia-induced apoptosis in Tca8113 cells is inhibited by heat shock protein 27 through blocking phospholipid scramblase 3 phosphorylation.; Authors - Jiang Wen W
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen ; KD Validated