Perilipin 1 Polyklonaler Antikörper

Perilipin 1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27716-1-AP

Synonyme

PERI, perilipin, Perilipin 1, PLIN, PLIN1

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Galerie der Validierungsdaten

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (Perilipin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (PLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, Mauslebergewebe, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: braunes Mausfettgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

27716-1-AP bindet in WB, IF, ELISA Perilipin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Perilipin 1 fusion protein Ag26426
• Vollständiger Name: perilipin
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 62-67 kDa
• GenBank-Zugangsnummer: NM_001145311
• Gene symbol: PLIN
• Gene ID (NCBI): 5346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and is a modulator of adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that derives in an abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

Publikationen

Species	Application	Title
mouse	WB	Hum Gene Ther; Kallistatin Improves High-Fat-Induced Insulin Resistance via Epididymal Adipose Tissue-Derived Exosomes; Authors - Zi-Wei Yang
human,mouse	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du