PLEKHA1 Polyklonaler Antikörper

PLEKHA1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26830-1-AP

Synonyme

PLEKHA1, TAPP 1, TAPP1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 26830-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 26830-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26830-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26830-1-AP

  IP result of anti-PLEKHA1 (IP:26830-1-AP, 4ug; Detection:26830-1-AP 1:1000) with A549 cells lysate 1600 ug.

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  IHC staining of human colon using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 26830-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26830-1-AP bindet in WB, IP, IHC, IF, ELISA PLEKHA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLEKHA1 fusion protein Ag25285
• Vollständiger Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC001136
• Gene symbol: PLEKHA1
• Gene ID (NCBI): 59338
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."