PLEK Monoklonaler Antikörper

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• KD/KO Validated

PLEK Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5C2

Kat-Nr. : CL488-66431

Synonyme

FLJ27168, P47, Platelet 47 kDa protein, pleckstrin, PLEK

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Galerie der Validierungsdaten

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  WB analysis using CL488-66431

  HL-60 cells were subjected to SDS PAGE followed by western blot with CL488-66431 (PLEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, THP-1-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66431 bindet in WB PLEK und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLEK fusion protein Ag3187
• Vollständiger Name: pleckstrin
• Berechnetes Molekulargewicht: 350 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC018549
• Gene symbol: PLEK
• Gene ID (NCBI): 5341
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)