Phospholipase C Beta 1 Polyklonaler Antikörper

Phospholipase C Beta 1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 26551-1-AP

Synonyme

KIAA0581, Phospholipase C beta 1, Phospholipase C I, PI PLC, PLC 154, PLC beta 1, PLC I, PLC154, PLCB1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 26551-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26551-1-AP (Phospholipase C beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26551-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human gliomas using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 26551-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

26551-1-AP bindet in WB, IHC, ELISA Phospholipase C Beta 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Phospholipase C Beta 1 fusion protein Ag24750
• Vollständiger Name: phospholipase C, beta 1 (phosphoinositide-specific)
• Berechnetes Molekulargewicht: 1216 aa, 139 kDa
• Beobachtetes Molekulargewicht: 139 kDa
• GenBank-Zugangsnummer: BC069420
• Gene symbol: PLCB1
• Gene ID (NCBI): 23236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLCB1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. PLCB1 is activated by two G-protein alpha subunits, alpha-q and alpha-11.

Publikationen

Species	Application	Title
human	WB	Biochem Genet; PLCB1 Enhances Cell Migration and Invasion in Gastric Cancer Via Regulating Actin Cytoskeletal Remodeling and Epithelial-Mesenchymal Transition; Authors - Yulin Wang ; KD Validated
human	WB	Front Endocrinol (Lausanne); Estrogen receptor subtype mediated anti-inflammation and vasorelaxation via genomic and nongenomic actions in septic mice; Authors - Luyun Zhang
human	WB,IHC	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated