uPA/Urokinase Polyklonaler Antikörper

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• KD/KO Validated

uPA/Urokinase Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Kat-Nr. : 17968-1-AP

Synonyme

ATF, PLAU, U plasminogen activator, uPA, uPA/Urokinase, URK

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Galerie der Validierungsdaten

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  WB analysis using 17968-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17968-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17968-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17968-1-AP

  IP Result of anti-uPA (IP:17968-1-AP, 4ug; Detection:17968-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17968-1-AP

  Immunofluorescent analysis of HeLa cells using 17968-1-AP (uPA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, A375-Zellen, HeLa-Zellen, humanes Hirngewebe, LNCaP-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Lungengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 29 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

17968-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA uPA/Urokinase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: uPA/Urokinase fusion protein Ag11813
• Vollständiger Name: plasminogen activator, urokinase
• Berechnetes Molekulargewicht: 431 aa, 49 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC013575
• Gene symbol: PLAU
• Gene ID (NCBI): 5328
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing. Defects in PLAU are the cause of Quebec platelet disorder (QPD).

Publikationen

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
mouse	WB	Gastroenterology; Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9-mediated Cytokine Release From Myeloid Cells.; Authors - Shinya Munakata
mouse	IF, WB	J Exp Med; PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.; Authors - Yu-Wen Cheng
human	IHC	Aging (Albany NY); Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.; Authors - Xingwang Zhou
mouse	WB	Angiogenesis; PDGFD switches on stem cell endothelial commitment.; Authors - Weisi Lu
human	WB	Aging (Albany NY); NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.; Authors - Pingping Ma