iPLA2 Polyklonaler Antikörper

iPLA2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 22030-1-AP

Synonyme

CaI PLA2, Group VI phospholipase A2, GVI, GVI PLA2, INAD1, iPLA2, iPLA2 beta, IPLA2 VIA, PARK14, PLA2, PLA2G6, PNPLA9

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 22030-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22030-1-AP (iPLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22030-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22030-1-AP (iPLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 22030-1-AP

  IP Result of anti-iPLA2 (IP:22030-1-AP, 4ug; Detection:22030-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IHC staining of human small intestine using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

22030-1-AP bindet in WB, IP, IHC, IF, ELISA iPLA2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: iPLA2 fusion protein Ag16896
• Vollständiger Name: phospholipase A2, group VI (cytosolic, calcium-independent)
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 88 kDa
• GenBank-Zugangsnummer: BC036742
• Gene symbol: PLA2G6
• Gene ID (NCBI): 8398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G6, also named as PLPLA9, is involved in the remodeling of membranes allowing arachidonic acid to be placed in the proper position in phospholipids for stimulated release. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. This protein has 4 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cell Rep; Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition; Authors - Laura Mahoney-Sanchez
human,mouse	WB,IHC	J Agric Food Chem; Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.; Authors - Shuhua Shan
rat	IF	Neurol Res; Calcium-independent phospholipase A2 inhibitor produces an analgesic effect in a rat model of neuropathic pain by reducing central sensitization in the dorsal horn.; Authors - Young Seob Gwak
human	IHC	Zhongguo Dang Dai Er Ke Za Zhi; [Clinical features of infantile neuroaxonal dystrophy and PLA2G6 gene testing].; Authors - Yao Lu
human	WB,IHC	Theranostics; Ferritin light chain promotes the reprogramming of glioma immune microenvironment and facilitates glioma progression; Authors - Hongjiang Li
human	IHC	Molecules; Identification of Diagnostic Metabolic Signatures in Thyroid Tumors Using Mass Spectrometry Imaging; Authors - Xinxin Mao