PLA2G4E Polyklonaler Antikörper

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• KD/KO Validated

PLA2G4E Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18088-1-AP

Synonyme

phospholipase A2, group IVE, PLA2G4E, PLA2G4E Ens

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 18088-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 18088-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 18088-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maus-Thymusgewebe, SW 1990-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

18088-1-AP bindet in WB, IHC, IF, ELISA PLA2G4E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLA2G4E fusion protein Ag12808
• Vollständiger Name: phospholipase A2, group IVE
• Berechnetes Molekulargewicht: 868 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99 kDa, 57 kDa
• GenBank-Zugangsnummer: BC101584
• Gene symbol: PLA2G4E
• Gene ID (NCBI): 123745
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

Publikationen

Species	Application	Title
mouse	WB	Autophagy; Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.; Authors - Junsheng Lou ; KD Validated
human,mouse	WB,IHC,IF	Prog Neurobiol; PLA2G4E, a candidate gene for resilience in Alzheimer´s disease and a new target for dementia treatment.; Authors - Marta Pérez-González ; KD Validated
mouse	WB	FASEB J; Group IVE cytosolic phospholipase A2 limits psoriatic inflammation by mobilizing the anti-inflammatory lipid N-acylethanolamine.; Authors - Luyiyun Liang
mouse	WB	Front Genet; Deletion of the Capn1 Gene Results in Alterations in Signaling Pathways Related to Alzheimer's Disease, Protein Quality Control and Synaptic Plasticity in Mouse Brain.; Authors - Wenyue Su
mouse	WB	J Neuroinflammation; Elamipretide alleviates pyroptosis in traumatically injured spinal cord by inhibiting cPLA2-induced lysosomal membrane permeabilization; Authors - Haojie Zhang