PKMYT1 Polyklonaler Antikörper

PKMYT1 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 55098-1-AP

Synonyme

MYT1, Myt1 kinase, PKMYT1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55098-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55098-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 55098-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55098-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 55098-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 55098-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A375-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Maushautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

55098-1-AP bindet in WB, ELISA PKMYT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase, membrane associated tyrosine/threonine 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_004203
• Gene symbol: PKMYT1
• Gene ID (NCBI): 9088
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)

Publikationen

Species	Application	Title
human	WB	Thorac Cancer; Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3; Authors - Dong Zhou