PI3 Kinase p85 Beta Monoklonaler Antikörper

PI3 Kinase p85 Beta Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2B3B6

Kat-Nr. : 67644-1-Ig

Synonyme

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2

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Galerie der Validierungsdaten

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  WB analysis using 67644-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PI3 Kinase p85 Beta antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67644-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PIK3R2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 67644-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67644-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, 4T1-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67644-1-Ig bindet in WB, IHC, IF, ELISA PI3 Kinase p85 Beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p85 Beta fusion protein Ag14369
• Vollständiger Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Berechnetes Molekulargewicht: 728 aa, 82 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC014170
• Gene symbol: PIK3R2
• Gene ID (NCBI): 5296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

Publikationen

Species	Application	Title
rat	WB	J Ethnopharmacol; Longdan Xiegan Tang attenuates liver injury and hepatic insulin resistance by regulating the angiotensin-converting enzyme 2/Ang (1-7)/Mas axis-mediated anti-inflammatory pathway in rats.; Authors - Chengliang Wang
human	IHC	Clin Transl Oncol; Anoikis patterns via machine learning strategy and experimental verification exhibit distinct prognostic and immune landscapes in melanoma; Authors - Jinfang Liu
mouse	WB	Environ Toxicol; Triclosan regulates the Nrf2/HO-1 pathway through the PI3K/Akt/JNK signaling cascade to induce oxidative damage in neurons.; Authors - Dan Wang
rat	WB,IF	Proteome Sci; TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats.; Authors - Yiming Xia