PI3 Kinase p110 Alpha Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PI3 Kinase p110 Alpha Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Kaninchen, Maus, Ratte und mehr (3)

Anwendung

WB, RIP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

79

CloneNo.

1F6A7

Rezensionen (1)

Kat-Nr. : 67071-1-Ig

Synonyme

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110 (alpha), PI3K p110(alpha), PIK3CA, PtdIns 3 kinase subunit alpha

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Galerie der Validierungsdaten

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  WB analysis of U2OS using 67071-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PI3 Kinase p110 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of pig brain using 67071-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PIK3CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67071-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67071-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67071-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PI3 Kinase p110 Alpha antibody (67071-1-Ig, Clone: 1F6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Jurkat using 67071-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PI3 Kinase p110 Alpha (67071-1-Ig, Clone:1F6A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Kaninchenhirngewebe, LNCaP-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 76 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

67071-1-Ig bindet in WB, RIP, IHC, IF, FC, CoIP, ELISA PI3 Kinase p110 Alpha und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Fisch, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p110 Alpha fusion protein Ag17392
• Vollständiger Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Berechnetes Molekulargewicht: 1068 aa, 124 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC113601
• Gene symbol: PIK3CA
• Gene ID (NCBI): 5290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publikationen

Species	Application	Title
human	WB	J Hazard Mater; Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.; Authors - Jing Qin
mouse	IHC	Cancer Lett; PI3K inhibitor impairs tumor progression and enhances sensitivity to anlotinib in anlotinib-resistant osteosarcoma.; Authors - Chenglong Chen
human	WB	Oxid Med Cell Longev; Transcriptional Patterns of Lower-Grade Glioma Patients with Distinct Ferroptosis Levels, Immunotherapy Response, and Temozolomide Sensitivity.; Authors - Zewei Tu
mouse	WB	Oxid Med Cell Longev; Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 Mice; Authors - Rui Li
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
rat	WB	Sci Total Environ; PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.; Authors - Mei Ha

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (02-18-2021)

Not sure what the band is, I saw a clear band in between 38 and 50KD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Fallopian tube epithelial cells

PI3 Kinase p110 Alpha Antibody Western Blot validation (1:2000 dilution) in Fallopian tube epithelial cells (Cat no:67071-1-Ig)