PIH1D1 Polyklonaler Antikörper

PIH1D1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 19427-1-AP

Synonyme

NOP17, Nucleolar protein 17 homolog, PIH1 domain containing 1, PIH1D1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 19427-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19427-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 19427-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 19427-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 19427-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 19427-1-AP

  human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19427-1-AP

  IP Result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human lung using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19427-1-AP

  Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, humanes Hodengewebe, humanes Uterusgewebe, Maus-Augengewebe, Maus-Eierstockgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

19427-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA PIH1D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIH1D1 fusion protein Ag13759
• Vollständiger Name: PIH1 domain containing 1
• Berechnetes Molekulargewicht: 290 aa, 32 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC001108
• Gene symbol: PIH1D1
• Gene ID (NCBI): 55011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.

Publikationen

Species	Application	Title
mouse	WB,IHC	Nat Commun; The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.; Authors - Chloé Maurizy
rat	WB	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
human		RNA; Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.; Authors - Machado-Pinilla Rosario R
human	WB	Cell Chem Biol; RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.; Authors - Paul Yenerall
mouse	WB	Chromosoma; The PRDM9 KRAB domain is required for meiosis and involved in protein interactions.; Authors - Yukiko Imai
mouse	WB	J Biol Chem; Nrf1-mediated transcriptional regulation of the proteasome requires a functional TIP60 complex.; Authors - Janakiram R Vangala