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  • KD/KO Validated

PIBF1 Polyklonaler Antikörper

PIBF1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14413-1-AP

Synonyme

C13orf24, CEP90, KIAA1008, PIBF, PIBF1, RP11 505F3.1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IFHEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14413-1-AP bindet in WB, IP, IF, ELISA PIBF1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PIBF1 fusion protein Ag5755
Vollständiger Name progesterone immunomodulatory binding factor 1
Berechnetes Molekulargewicht 90 kDa
Beobachtetes Molekulargewicht 90 kDa
GenBank-ZugangsnummerBC051911
Gene symbol PIBF1
Gene ID (NCBI) 10464
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.

Protokolle

Produktspezifische Protokolle
WB protocol for PIBF1 antibody 14413-1-APProtokoll herunterladen
IF protocol for PIBF1 antibody 14413-1-APProtokoll herunterladen
IP protocol for PIBF1 antibody 14413-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB, IP, IF

Elife

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Authors - Andrew Kodani
  • KD Validated
humanWB

J Cell Biol

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

Authors - Dhivya Kumar
  • KO Validated
humanIF

EMBO Rep

Zika virus alters centrosome organization to suppress the innate immune response.

Authors - Andrew Kodani
humanIF

PLoS Biol

The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly

Authors - Pierrick Le Borgne
  • KD Validated
humanIF

Cell Rep

CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

Authors - H Kubra Gurkaslar
humanIF

EMBO J

Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis

Authors - Marine H Laporte

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elisa (Verified Customer) (06-20-2022)

HEK293 cells stained for Hoechst (DNA marker, in green), Cep90 (mother centriole distal appendage marker, in magenta) and PCNT (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 2' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilisation was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). Cep90 antibody recognises clearly dots at the centrosome (identified by the presence of PCNT).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: HEK293 cells (Human embryonic kidney 293 cells)
PIBF1 Antibody Immunofluorescence validation (1:300 dilution) in HEK293 cells (Human embryonic kidney 293 cells) (Cat no:14413-1-AP)
FH

Pierrick (Verified Customer) (10-24-2019)

Antibody mostly used in wester blot and work well at 1/1000 dilution on RPE1 and HEK293 sample

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: PIBF1 rabbit: if 1/500 WB 1/1000
  • Cell Tissue Type: RPE1, HEK290, HeLa Kyoto