- Featured Product
- KD/KO Validated
PIBF1 Polyklonaler Antikörper
PIBF1 Polyklonal Antikörper für IF, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IP, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14413-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen |
Erfolgreiche IP | HEK-293-Zellen |
Erfolgreiche Detektion in IF | HEK-293-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 2 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
Produktinformation
14413-1-AP bindet in WB, IP, IF, ELISA PIBF1 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | PIBF1 fusion protein Ag5755 |
Vollständiger Name | progesterone immunomodulatory binding factor 1 |
Berechnetes Molekulargewicht | 90 kDa |
Beobachtetes Molekulargewicht | 90 kDa |
GenBank-Zugangsnummer | BC051911 |
Gene symbol | PIBF1 |
Gene ID (NCBI) | 10464 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.
Protokolle
Produktspezifische Protokolle | |
---|---|
WB protocol for PIBF1 antibody 14413-1-AP | Protokoll herunterladen |
IF protocol for PIBF1 antibody 14413-1-AP | Protokoll herunterladen |
IP protocol for PIBF1 antibody 14413-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Elife Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
| ||
J Cell Biol A ciliopathy complex builds distal appendages to initiate ciliogenesis.
| ||
EMBO Rep Zika virus alters centrosome organization to suppress the innate immune response. | ||
PLoS Biol The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly
| ||
Cell Rep CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression. | ||
EMBO J Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elisa (Verified Customer) (06-20-2022) | HEK293 cells stained for Hoechst (DNA marker, in green), Cep90 (mother centriole distal appendage marker, in magenta) and PCNT (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 2' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilisation was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). Cep90 antibody recognises clearly dots at the centrosome (identified by the presence of PCNT).
|
FH Pierrick (Verified Customer) (10-24-2019) | Antibody mostly used in wester blot and work well at 1/1000 dilution on RPE1 and HEK293 sample
|