PIAS4 Polyklonaler Antikörper

PIAS4 Polyklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18469-1-AP

Synonyme

E3 SUMO protein ligase PIAS4, PIAS gamma, PIAS4, Piasg, PIASY, ZMIZ6

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 18469-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18469-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18469-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 18469-1-AP (PIAS4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18469-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PIAS4 (18469-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18469-1-AP bindet in WB, IF, FC, ELISA PIAS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIAS4 fusion protein Ag13316
• Vollständiger Name: protein inhibitor of activated STAT, 4
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-70 kDa
• GenBank-Zugangsnummer: BC010047
• Gene symbol: PIAS4
• Gene ID (NCBI): 51588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members(PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).