PHLPPL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PHLPPL Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 25244-1-AP

Synonyme

KIAA0931, PHLPP like, PHLPP2, PHLPPL

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Galerie der Validierungsdaten

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  WB analysis using 25244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25244-1-AP

  WB result of PHLPPL antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPPL transfected HeLa cells.

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  IP experiment of HeLa using 25244-1-AP

  IP result of anti-PHLPPL(IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug.

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  IHC staining of human colon cancer using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 25244-1-AP

  Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPPL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

25244-1-AP bindet in WB, IP, IHC, IF, ELISA PHLPPL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHLPPL fusion protein Ag19000
• Vollständiger Name: PH domain and leucine rich repeat protein phosphatase-like
• Berechnetes Molekulargewicht: 1323 aa, 147 kDa
• Beobachtetes Molekulargewicht: 147 kDa
• GenBank-Zugangsnummer: BC129927
• Gene symbol: PHLPPL
• Gene ID (NCBI): 23035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHLPPL also named as KIAA0931 or PHLPP2 is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.

Publikationen

Species	Application	Title
mouse	WB,IF	Oxid Med Cell Longev; Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway.; Authors - De-Feng Li ; KD Validated
human,mouse	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human	WB	Oncotarget; PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.; Authors - Alena J Smith ; KD Validated
human	WB,IHC	Oncotarget; High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.; Authors - Youyou Xie
zebrafish	WB	FASEB J; Role of epigenetic m6 A RNA methylation in vascular development: mettl3 regulates vascular development through PHLPP2/mTOR-AKT signaling.; Authors - Ramendu Parial
human	WB, IHC	Front Oncol; Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.; Authors - Wei Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Slavica (Verified Customer) (10-04-2021)

The antibody worked very well!

• Applications: Western Blot
• Cell Tissue Type: SHED patient cell lines