PHLDA1 Polyklonaler Antikörper

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• KD/KO Validated

PHLDA1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 18263-1-AP

Synonyme

DT1P1B11, PHLDA1, PHRIP, PQ rich protein, PQR protein, TDAG51

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Galerie der Validierungsdaten

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  WB analysis using 18263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18263-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 18263-1-AP

  Immunofluorescent analysis of A375 cells using 18263-1-AP (PHLDA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, BxPC-3-Zellen, HeLa-Zellen, Maushirngewebe, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IF: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

18263-1-AP bindet in WB, IHC, IF, ELISA PHLDA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHLDA1 fusion protein Ag13125
• Vollständiger Name: pleckstrin homology-like domain, family A, member 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC018929
• Gene symbol: PHLDA1
• Gene ID (NCBI): 22822
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Brain Behav Immun; PHLDA1 promotes microglia-mediated neuroinflammation via regulating K63-linked ubiquitination of TRAF6.; Authors - Chaojun Han ; KD Validated
rat	WB	Life Sci; PHLDA1 is a new therapeutic target of oxidative stress and ischemia reperfusion-induced myocardial injury.; Authors - Yuxuan Guo ; KD Validated
mouse	WB	Inflammation; TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT-GSK-3β Pathway.; Authors - Chuntian Liu ; KD Validated
mouse	WB	Hum Exp Toxicol; Loss of PHLDA1 has a protective role in OGD/R-injured neurons via regulation of the GSK-3β/Nrf2 pathway.; Authors - F Yang ; KD Validated
human	WB,IHC,IF	Cell Death Dis; The long non-coding RNA SNHG1 promotes glioma progression by competitively binding to miR-194 to regulate PHLDA1 expression.; Authors - Liang Liu ; KD Validated
human	WB	Mol Ther Nucleic Acids; lncRNA HIF1A Antisense RNA 2 Modulates Trophoblast Cell Invasion and Proliferation through Upregulating PHLDA1 Expression.; Authors - Dan Wu