PHF17 Polyklonaler Antikörper

PHF17 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15032-1-AP

Synonyme

JADE1, KIAA1807, PHD finger protein 17, PHF17, Protein Jade 1

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 15032-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15032-1-AP (PHF17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15032-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15032-1-AP (PHF17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Produktinformation

15032-1-AP bindet in WB, IP, IHC, ELISA PHF17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF17 fusion protein Ag4290
• Vollständiger Name: PHD finger protein 17
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 58-61 kDa, 94-96 kDa
• GenBank-Zugangsnummer: BC032376
• Gene symbol: PHF17
• Gene ID (NCBI): 79960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHF17 was identified as a protein partner of the von Hippel-Lindau tumor suppressor pVHL. It is one component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It can act as a transcriptional coactivator that promote acetylation of nucleosomal histone H4 by KAT5. In addition, it ubiquitinates both phosphorylated and non-phosphorylated β-catenin and therefore regulates canonical Wnt signaling in both Wnt-off and Wnt-on phases. PHF17 exist several isoforms with molecular weight 100-110 and 55-65 kDa.

Publikationen

Species	Application	Title
human	WB,IHC,IP	Acta Neuropathol; Genome-wide association study and functional validation implicates JADE1 in tauopathy.; Authors - Kurt Farrell
human	WB	J Biol Chem; The ciliary protein nephrocystin-4 translocates the canonical Wnt regulator Jade-1 to the nucleus to negatively regulate β-catenin signaling.; Authors - Borgal Lori L
human	WB, IP	J Biol Chem; Casein Kinase 1 Alpha Phosphorylates the Wnt-Regulator Jade-1 and Modulates its Activity.; Authors - Lori Borgal
human	WB	Cell Cycle; Jade-1S phosphorylation induced by CK1α contributes to cell cycle progression.; Authors - Lori Borgal
human	WB	J Toxicol Sci; Involvement of microRNA-4680-3p against phenytoin-induced cell proliferation inhibition in human palate cells; Authors - Yosuke Tsukiboshi