PHF10 Monoklonaler Antikörper

PHF10 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

4D4C6

Kat-Nr. : 66341-1-Ig

Synonyme

BAF45a, BRG1 associated factor 45a, PHD finger protein 10, PHF10, XAP135

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Galerie der Validierungsdaten

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  WB analysis of fetal human brain using 66341-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66341-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66341-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66341-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 66341-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66341-1-Ig (PHF10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66341-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66341-1-Ig (PHF10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, HEK-293-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66341-1-Ig bindet in WB, IHC, IF, CoIP, ELISA PHF10 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PHF10 fusion protein Ag19096
• Vollständiger Name: PHD finger protein 10
• Berechnetes Molekulargewicht: 408 aa, 46 kDa
• Beobachtetes Molekulargewicht: 56 kDa, 35 kDa
• GenBank-Zugangsnummer: BC020954
• Gene symbol: PHF10
• Gene ID (NCBI): 55274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHF10, also named as BRG1-associated factor 45a, is a 498 amino acid protein, which locate in the nucleus and Belongs to the SAYP family. PHF10 Involve in transcription activity regulation by chromatin remodeling. It Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. PHF10 exists as several isoform and the calculated molecular weight of each isoform is 42 kDa, 37 kDa, 51 kDa, and 56 kDa.

Publikationen

Species	Application	Title
mouse	WB	Glia; DHHC5 facilitates oligodendrocyte development by palmitoylating and activating STAT3.; Authors - Yanchen Ma
human	CoIP	bioRxiv; BRD9-SMAD2/3 orchestrates stemness and tumorigenesis in pancreatic ductal adenocarcinoma; Authors - Yuliang Feng
human,mouse	IF	Gastroenterology; BRD9-SMAD2/3 orchestrates stemness and tumorigenesis in pancreatic ductal adenocarcinoma; Authors - Yuliang Feng