PGK2 Monoklonaler Antikörper

PGK2 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3D4F2

Kat-Nr. : 68228-1-Ig

Synonyme

dJ417L20.2, PGK 2, PGK2, PGKB, PGKPS, phosphoglycerate kinase 2

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Galerie der Validierungsdaten

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  WB analysis using 68228-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68228-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 68228-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 68228-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (68228-1-Ig, Clone: 3D4F2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of A549 using 68228-1-Ig

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human PGK2 (68228-1-Ig, Clone:3D4F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Herzgewebe, HuH-7-Zellen, humanes Hodengewebe, MOLT-4-Zellen, Raji-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IF: Maushodengewebe
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68228-1-Ig bindet in WB, IF, FC, ELISA PGK2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PGK2 fusion protein Ag20631
• Vollständiger Name: phosphoglycerate kinase 2
• Berechnetes Molekulargewicht: 417 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC038843
• Gene symbol: PGK2
• Gene ID (NCBI): 5232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.