PGD Monoklonaler Antikörper

PGD Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10G4

Kat-Nr. : 67916-1-Ig

Synonyme

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

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Galerie der Validierungsdaten

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  WB analysis using 67916-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67916-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67916-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PGD antibody (67916-1-Ig, Clone: 2A10G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67916-1-Ig bindet in WB, IF, ELISA PGD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PGD fusion protein Ag6871
• Vollständiger Name: phosphogluconate dehydrogenase
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 45 kDa
• GenBank-Zugangsnummer: BC000368
• Gene symbol: PGD
• Gene ID (NCBI): 5226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)