PGCP Polyklonaler Antikörper

PGCP Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16601-1-AP

Synonyme

CPQ, LCH1, Lysosomal dipeptidase, PGCP, plasma glutamate carboxypeptidase

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Galerie der Validierungsdaten

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  WB analysis of L02 using 16601-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 16601-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16601-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16601-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16601-1-AP (PGCP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16601-1-AP

  IP Result of anti-PGCP (IP:16601-1-AP, 4ug; Detection:16601-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IF Staining of L02 using 16601-1-AP

  Immunofluorescent analysis of L02 cells using 16601-1-AP (PGCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, humanes Plasmagewebe, humanes Plazenta-Gewebe, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IF: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16601-1-AP bindet in WB, IP, IF, ELISA PGCP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGCP fusion protein Ag9770
• Vollständiger Name: plasma glutamate carboxypeptidase
• Berechnetes Molekulargewicht: 472 aa, 52 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC020689
• Gene symbol: PGCP
• Gene ID (NCBI): 10404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGCP(plasma glutamate carboxypeptidase) is also named as LCH1,CPQ and belongs to the peptidase M28 family. It may play an important role in the hydrolysis of circulating peptides. It catalyzes more efficiently the hydrolysis of dipeptides with unsubstituted terminals into amino acids. It is a proteinase that acts on the unsubstituted N- and C-termini of dipeptides. It has been suggested that this PGCP is involved in the release of thyroxine(PMID:20951214). Human PGCP is a metallopeptidase with five potential glycosylation sites, whose activity depends on dimerizationand it can be detected a band of 65-72kd in FRTL-5 cells which is pro-PGCP(PMID:22127294). It can be N-glycosylated(PMID:10206990). It has the pro-form of 60-65 kDa and mature form of 55 kDa(PMID:20951214).

Publikationen

Species	Application	Title
human,rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li