PGAP1 Polyklonaler Antikörper

PGAP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 55392-1-AP

Synonyme

Bst1, GPI inositol deacylase, hPGAP1, ISPD3024, PGAP1

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Galerie der Validierungsdaten

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  WB analysis of human testis using 55392-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 55392-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 55392-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55392-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55392-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55392-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PGAP1 antibody (55392-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, HEK-293-Zellen, Maushodengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

55392-1-AP bindet in WB, IHC, IF, ELISA PGAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: post-GPI attachment to proteins 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 49-53 kDa, 85-90 kDa
• GenBank-Zugangsnummer: NM_024989
• Gene symbol: PGAP1
• Gene ID (NCBI): 80055
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells; Authors - Tatsuaki Kurosaki
human	WB	J Biol Chem; Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.; Authors - Takahiro Nakane

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

pitna (Verified Customer) (12-19-2019)

PGAP1According to Uniprot, this protein has 4 isoforms ranging from 49 – 105 KDa. it appears that the regulation of the different isoforms is still an open question in the literatureTo reduce non-specific binding, blots were incubated in Odyssey Blocking buffer (Li-Cor) for 1 hour at room temperature (RT) .

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)

PGAP1 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:55392-1-AP)