PEX14 (human specific) Rekombinanter Antikörper

PEX14 (human specific) Rekombinant Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

6K5

Kat-Nr. : 80196-1-RR

Synonyme

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, PEX14 (human specific), Pex14p, PTS1 receptor docking protein

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Galerie der Validierungsdaten

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  WB analysis using 80196-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 80196-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 80196-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX14 antibody (80196-1-RR, Clone: 6K5 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 80196-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (human specific) (80196-1-RR, Clone:6K5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80196-1-RR bindet in WB, IHC, IF, FC, ELISA PEX14 (human specific) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PEX14 (human specific) fusion protein Ag0932
• Vollständiger Name: peroxisomal biogenesis factor 14
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC006327
• Gene symbol: PEX14
• Gene ID (NCBI): 5195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

This antibody is specific to human PEX14. It can't detect mouse or rat PEX14.