PELP1 Monoklonaler Antikörper

PELP1 Monoklonal Antikörper für FC (Intra), IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1B11E4

Rezensionen (1)

Kat-Nr. : 67050-1-Ig

Synonyme

HMX3, MNAR, P160, PELP1, Transcription factor HMX3

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Galerie der Validierungsdaten

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  WB analysis using 67050-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 67050-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67050-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 67050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of MCF-7 using 67050-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67050-1-Ig bindet in WB, IHC, IF, FC (Intra), ELISA PELP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PELP1 fusion protein Ag25729
• Vollständiger Name: proline, glutamate and leucine rich protein 1
• Berechnetes Molekulargewicht: 1130 aa, 120 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC069058
• Gene symbol: PELP1
• Gene ID (NCBI): 27043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.

Publikationen

Species	Application	Title
mouse	WB,IHC	Int Immunopharmacol; MiR-195-5p is involved in testicular ischemia/reperfusion injury by directly targeting PELP1 and regulating spermatogonia pyroptosis; Authors - Kai-Xiang He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

fatemeh (Verified Customer) (11-30-2021)

The antibody works well on human protein lystae (30 ug of protein from Hek293 cells, 8% gel, wet transfer 90min 300mA in tris-Gly buffer on PVDF, incubation with primary ab was onvernight at 4C). we coulnt see the specific band for mouse cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1-500
• Cell Tissue Type: Hek293 (human), E14 (mouse)

PELP1 Antibody Western Blot validation (1-500 dilution) in Hek293 (human), E14 (mouse) (Cat no:67050-1-Ig)