PEDF Polyklonaler Antikörper

PEDF Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 26045-1-AP

Synonyme

EPC 1, PEDF, PIG35, Serpin F1, SERPINF1

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Galerie der Validierungsdaten

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  WB analysis of human serum using 26045-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 26045-1-AP (PEDF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 26045-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26045-1-AP (PEDF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, human serum

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

26045-1-AP bindet in WB, IF, ELISA PEDF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEDF fusion protein Ag23296
• Vollständiger Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• Berechnetes Molekulargewicht: 418 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC013984
• Gene symbol: SERPINF1
• Gene ID (NCBI): 5176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

Publikationen

Species	Application	Title
human	WB,IF	J Cell Mol Med; Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization; Authors - Lele Li
human	WB	Hum Gene Ther; Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor.; Authors - Hao Zhang
human	WB	Cell Death Dis; TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway.; Authors - Xiaoyan Fu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (05-14-2021)

very good for WB

• Applications: Western Blot