PECR Polyklonaler Antikörper

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• KD/KO Validated

PECR Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14901-1-AP

Synonyme

DCR RP, HPDHase, HSA250303, PECR, pVI ARL, SDR29C1, TERP

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Galerie der Validierungsdaten

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  WB analysis using 14901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14901-1-AP (PECR antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14901-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14901-1-AP (PECR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14901-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14901-1-AP (PECR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14901-1-AP

  IP result of anti-PECR(IP:14901-1-AP, 4ug; Detection:14901-1-AP 1:2000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of human liver cancer using 14901-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14901-1-AP (PECR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14901-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14901-1-AP (PECR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Hodengewebe, humanes Nierengewebe, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

14901-1-AP bindet in WB, IP, IHC, ELISA PECR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PECR fusion protein Ag6693
• Vollständiger Name: peroxisomal trans-2-enoyl-CoA reductase
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC002529
• Gene symbol: PECR
• Gene ID (NCBI): 55825
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisomal trans-2-enoyl-CoA reductase (PECR) is also called TERP and TECR, which is located on chromosome q35 with 86627 bases. It can participate in carbon chain elongation in fatty acid metabolism and catalyze the last reaction of four long chain fatty acid elongation cycles. Each cycle of PECR adds two carbons to the long chain and very long chain fatty acid (VLCFA) chains, reducing the intermediate of trans-2,3-enoyl coenzyme A fatty acid to acyl coenzyme A, which can further extend the carbon chain by entering a new elongation cycle. Meanwhile, PECR is a peroxisome protein involved in fatty acid synthesis and plays an important role in milk fat synthesis. The molecular mass of PECR is 33 kDa. (PMID: 31467878)

Publikationen

Species	Application	Title
human	WB,IHC	Acta Histochem; Peroxisomal trans-2-enoyl-CoA inhibits proliferation, migration and invasion of hepatocellular carcinoma cells; Authors - Qingqing Luo ; KD Validated