ERp57/ERp60 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ERp57/ERp60 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (1)

Kat-Nr. : 15967-1-AP

Synonyme

58 kDa microsomal protein, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER protein 57, ER protein 60, ER60, ERp57, ERp57/ERp60, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

---

Galerie der Validierungsdaten

• 

  WB analysis using 15967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15967-1-AP (ERp57/ERp60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15967-1-AP

  IP Result of anti-ERp57/ERp60 (IP:15967-1-AP, 4ug; Detection:15967-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 15967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (15967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 15967-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ERp57/ERp60 antibody (15967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 7 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

15967-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ERp57/ERp60 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERp57/ERp60 fusion protein Ag8741
• Vollständiger Name: protein disulfide isomerase family A, member 3
• Berechnetes Molekulargewicht: 505 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC014433
• Gene symbol: PDIA3
• Gene ID (NCBI): 2923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Publikationen

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
pig	WB	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
mouse	WB	Redox Biol; Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress.; Authors - Xie Ge
rat	IF	EMBO J; TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization.; Authors - Adam Johnson
human	IF	Cell Rep; Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.; Authors - Jeffrey R Jones
human	IF	J Clin Endocrinol Metab; Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.; Authors - Liu Xin-Mei XM

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Prasanna (Verified Customer) (05-07-2018)

Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: normal rat kidney cells