PDI Monoklonaler Antikörper

PDI Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

2E6A11

Rezensionen (1)

Kat-Nr. : 66422-1-Ig

Synonyme

DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

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Galerie der Validierungsdaten

• 

  WB analysis using 66422-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66422-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 66422-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, HepG2-Zellen, L02-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:100000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 12 publications below

Produktinformation

66422-1-Ig bindet in WB, IHC, IF, ELISA PDI und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDI fusion protein Ag1747
• Vollständiger Name: prolyl 4-hydroxylase, beta polypeptide
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC014504
• Gene symbol: P4HB
• Gene ID (NCBI): 5034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Publikationen

Species	Application	Title
human	IF	Biochim Biophys Acta Mol Cell Res; SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization; Authors - Wojciech Wiertelak
human	IF	J Cell Sci; A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function; Authors - Janine McCaughey
human	WB	Mol Pharm; Highly Efficient Method for Intracellular Delivery of Proteins Mediated by Cholera Toxin-Induced Protein Internalization.; Authors - Xiaofan Jia
human	IF	Cell Signal; HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression.; Authors - Liu Liu
human	IF	Biochim Biophys Acta Gen Subj; Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels; Authors - Tianmiao Huang
mouse	IF	Mol Med Rep; Sigma‑1 receptor overexpression promotes proliferation and ameliorates cell apoptosis in β‑cells.; Authors - Mengting Ke

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

10ug total protein of HEK293T lysate loaded. Membrane blocked in 5% BSA. Antibody (1:5,000) incubated overnight in block at 4 degrees. Anti-mouse HRP used at 1 in 10,000 to detect band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

PDI Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:66422-1-Ig)