PDHB Polyklonaler Antikörper

PDHB Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 14744-1-AP

Synonyme

PDHB, PDHE 1B, PDHE1 B, PDHE1B, PHE1B, Pyruvate Dehydrogenase E1 beta subunit

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Galerie der Validierungsdaten

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  WB analysis using 14744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14744-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14744-1-AP

  IP Result of anti-PDHB (IP:14744-1-AP, 4ug; Detection:14744-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 14744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14744-1-AP (PDHB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 14744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14744-1-AP (PDHB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14744-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDHB antibody (14744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, humanes Herzgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

14744-1-AP bindet in WB, IP, IHC, IF, ELISA PDHB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Fledermaus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDHB fusion protein Ag6442
• Vollständiger Name: pyruvate dehydrogenase (lipoamide) beta
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000439
• Gene symbol: PDHB
• Gene ID (NCBI): 5162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDHB(Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named as PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors -
human	WB	Mol Cell; Phosphorylation of PDHA by AMPK Drives TCA Cycle to Promote Cancer Metastasis.; Authors - Zhen Cai
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	Circ Res; (Pro)renin Receptor Inhibition Reprograms Hepatic Lipid Metabolism and Protects Mice from Diet-Induced Obesity and Hepatosteatosis.; Authors - Liwei Ren
mouse	WB	J Allergy Clin Immunol; Pyruvate dehydrogenase has a major role in mast cell function, and its activity is regulated by mitochondrial microphthalmia transcription factor.; Authors - Israa Sharkia