PDE6D Monoklonaler Antikörper

PDE6D Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H4B6

Rezensionen (2)

Kat-Nr. : 67565-1-Ig

Synonyme

GMP PDE delta, PDE6D, PDED, Protein p17

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 67565-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67565-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67565-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat retina using 67565-1-Ig

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67565-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67565-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, JAR-Zellen, Maushirngewebe, Rattenhirngewebe, Ratten-Retina-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67565-1-Ig bindet in WB, ELISA PDE6D und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDE6D fusion protein Ag29563
• Vollständiger Name: phosphodiesterase 6D, cGMP-specific, rod, delta
• Berechnetes Molekulargewicht: 17 KDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: NM_002601
• Gene symbol: PDE6D
• Gene ID (NCBI): 5147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Charlotte (Verified Customer) (07-24-2023)

We tested the antibody against PDE6 delta KO cells. We can see the antibody is very noisy but it is working.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: NIH-3T3

PDE6D Antibody Western Blot validation (1/2000 dilution) in NIH-3T3 (Cat no:67565-1-Ig)

FH

Criseyda (Verified Customer) (09-19-2022)

The antibody had background bands/nonspecific bands present but worked best in 5% Milk TBS-T (Blocking, Primary and Secondary) and not 3% BSA TBS-T (Blocking, Primary and Secondary).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T47D

PDE6D Antibody Western Blot validation (1:1000 dilution) in T47D (Cat no:67565-1-Ig)