PDE6A Polyklonaler Antikörper

PDE6A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21200-1-AP

Synonyme

CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA

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Galerie der Validierungsdaten

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  WB analysis using 21200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21200-1-AP (PDE6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye tissue using 21200-1-AP

  WB results of PDE6A antibody (21200-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

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  WB analysis of human brain using 21200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21200-1-AP (PDE6A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21200-1-AP (PDE6A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21200-1-AP (PDE6A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of retinal tissue using 21200-1-AP

  IF stain of PDE6a antibody with retinal tissue by a customer. (PDE6a is in red and nuclei are blue).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, humanes Hirngewebe, Ratten-Augengewebe
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, humanes Kolonkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Retina-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

21200-1-AP bindet in WB, IHC, IF, ELISA PDE6A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE6A fusion protein Ag15562
• Vollständiger Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
• Berechnetes Molekulargewicht: 860 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC035909
• Gene symbol: PDE6A
• Gene ID (NCBI): 5145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.

Publikationen

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
rat	WB	Toxicol Appl Pharmacol; Ocular toxicity of AUY922 in pigmented and albino rats.; Authors - Danielle Roman
mouse	WB	Dev Biol; Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.; Authors - Jeremy R Egbert
mouse	WB	PLoS One; Mouse models of human ocular disease for translational research.; Authors - Mark P Krebs