PDE5A Polyklonaler Antikörper

PDE5A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 22624-1-AP

Synonyme

CGB PDE, CN5A, PDE5, PDE5A, PDE5A1, phosphodiesterase 5A, cGMP

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Galerie der Validierungsdaten

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  WB analysis using 22624-1-AP

  mouse lung lysates were subjected to SDS PAGE followed by western blot with 22624-1-AP (PDE5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 22624-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22624-1-AP (PDE5A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 22624-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22624-1-AP (PDE5A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 22624-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 22624-1-AP (PDE5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 22624-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 22624-1-AP (PDE5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 22624-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 22624-1-AP (PDE5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

22624-1-AP bindet in WB, IHC, IF, ELISA PDE5A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE5A fusion protein Ag18779
• Vollständiger Name: phosphodiesterase 5A, cGMP-specific
• Berechnetes Molekulargewicht: 875 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC126233
• Gene symbol: PDE5A
• Gene ID (NCBI): 8654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE5A (cGMP-binding cGMP-specific phosphodiesterase) plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Through alternative splicing, the PDE5A gene produces three proteins (PDE5A1, PDE5A2, and PDE5A3) that differ in their N termini and range in mass from 89 to 105 kDa. (PMID: 25704994; 21215707). A second 70 kDa band was consistently observed in heart tissue that either reflected a splice variant or proteolytic fragment of PDE5A. (PMID: 15576651).

Publikationen

Species	Application	Title
	IF	Am J Respir Cell Mol Biol; Decreased Cyclic GMP-protein Kinase G signaling impairs Angiogenesis in a Lamb Model of Persistent Pulmonary Hypertension of Newborn.; Authors - Megha Sharma
bovine	WB	Front Cell Dev Biol; Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.; Authors - Mingjuan Gu
mouse	WB,IHC	Front Cell Dev Biol; An MRTF-A-Sp1-PDE5 Axis Mediates Angiotensin-II-Induced Cardiomyocyte Hypertrophy.; Authors - Teng Wu
rabbit	WB	Am J Physiol Gastrointest Liver Physiol; Augmentation of cGMP/PKG Pathway and Colonic Motility by Hydrogen Sulfide.; Authors - Ancy D Nalli
human	WB, IHC	PeerJ; Identification of potential genes in upper tract urothelial carcinoma using next-generation sequencing with bioinformatics and in vitro analyses.; Authors - Hsiang-Ying Lee
mouse	WB	J Ethnopharmacol; Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC (soluble guanylate cyclase) - cGMP (cyclic guanosine monophosphate) - PKG (protein kinase G) pathway; Authors - Liqian Chen