PDE4A Polyklonaler Antikörper

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• KD/KO Validated

PDE4A Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 16226-1-AP

Synonyme

DPDE2, PDE4, PDE46, PDE4A

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Galerie der Validierungsdaten

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  WB analysis of human brain using 16226-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16226-1-AP

  WB result of PDE4A antibody (16226-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDE4A transfected HeLa cells.

• 

  WB analysis of human brain using 16226-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16226-1-AP

  IP Result of anti-PDE4A (IP:16226-1-AP, 4ug; Detection:16226-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 16226-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16226-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of SH-SY5Y using 16226-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug PDE4A antibody (16226-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

16226-1-AP bindet in WB, IP, IHC, FC, ELISA PDE4A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE4A fusion protein Ag8018
• Vollständiger Name: phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila)
• Berechnetes Molekulargewicht: 98 kDa
• Beobachtetes Molekulargewicht: 125-130 kDa
• GenBank-Zugangsnummer: BC019864
• Gene symbol: PDE4A
• Gene ID (NCBI): 5141
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Br J Pharmacol; Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone.; Authors - Emma Mitidieri
mouse	WB	Int J Mol Sci; Phosphodiesterases Expression during Murine Cardiac Development.; Authors - Thays Maria da Conceição Silva Carvalho
mouse	WB	Biochem Pharmacol; DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.; Authors - Heng Li
human	WB,IHC	Cell Signal; Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.; Authors - Peter H Schafer
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu
human	IHC	Drugs R D; Apremilast Normalizes Gene Expression of Inflammatory Mediators in Human Keratinocytes and Reduces Antigen-Induced Atopic Dermatitis in Mice.; Authors - Peter H Schafer