PDE2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDE2A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 55306-1-AP

Synonyme

CGS PDE, cGSPDE, PDE2A, PDE2A1, PED2A4

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55306-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55306-1-AP

  IP Result of anti-PDE2A (IP:55306-1-AP, 4ug; Detection:55306-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

55306-1-AP bindet in WB, IP, IHC, IF, ELISA PDE2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phosphodiesterase 2A, cGMP-stimulated
• Berechnetes Molekulargewicht: 106 kDa
• Beobachtetes Molekulargewicht: 100-106 kDa
• GenBank-Zugangsnummer: NM_002599
• Gene symbol: PDE2A
• Gene ID (NCBI): 5138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE2A, also named as cGSPDE and CGS-PDE, belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. It is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. This antibody is specific to PDE2A.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.; Authors - Mehdi Eshraghi
rat	WB	Br J Pharmacol; 17β-estradiol reduces mitochondrial cAMP content and cytochrome oxidase activity in a phosphodiesterase 2-dependent manner.; Authors - Sofya Pozdniakova
rat	WB	Biochem Pharmacol; Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A.; Authors - Módis Katalin K
rat	WB	Front Pharmacol; Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress.; Authors - Xiaofei Zhu
human,mouse	WB,IF	Commun Biol; Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy.; Authors - Miguel J Lobo ; KO Validated
rat	WB	JCI Insight; Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity.; Authors - Kun Liu