PD-L2/CD273 Polyklonaler Antikörper

PD-L2/CD273 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 18251-1-AP

Synonyme

B7 DC, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, PD-L2, PD-L2/CD273, Programmed death ligand 2

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Galerie der Validierungsdaten

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  WB analysis using 18251-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18251-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 18251-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18251-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18251-1-AP (PD-L2/CD273 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HepG2-Zellen, Mausherzgewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 11 publications below

Produktinformation

18251-1-AP bindet in WB, IHC, ELISA PD-L2/CD273 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PD-L2/CD273 fusion protein Ag13039
• Vollständiger Name: programmed cell death 1 ligand 2
• Berechnetes Molekulargewicht: 273 aa, 31 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa, 28 kDa
• GenBank-Zugangsnummer: BC074766
• Gene symbol: PDCD1LG2
• Gene ID (NCBI): 80380
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery.

Publikationen

Species	Application	Title
human	WB,IHC	Aging Cell; Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.; Authors - Qixia Xu
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	IHC	Cancer Sci; ANO9 regulates PD-L2 expression and binding ability to PD-1 in gastric cancer.; Authors - Keita Katsurahara
human	IHC	J Cell Mol Med; IFI30 expression is an independent unfavourable prognostic factor in glioma.; Authors - Xiu Liu
human	WB	J Transl Med; Treatment with eFT-508 increases chemosensitivity in breast cancer cells by modulating the tumor microenvironment.; Authors - Zhao-Ying Yang
human	IHC	Oncol Rep; Differential expression of PD‑L1 and PD‑L2 is associated with the tumor microenvironment of TILs and M2 TAMs and tumor differentiation in non‑small cell lung cancer.; Authors - Ryota Sumitomo