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PCSK9 Polyklonaler Antikörper

PCSK9 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27882-1-AP

Synonyme

FH3, HCHOLA3, LDLCQ1, NARC 1, NARC1, PC9, PCSK9, Proprotein convertase 9



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27882-1-AP bindet in WB, IHC, IF, FC, ELISA PCSK9 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PCSK9 fusion protein Ag27254
Vollständiger Name proprotein convertase subtilisin/kexin type 9
Berechnetes Molekulargewicht 74 kDa
Beobachtetes Molekulargewicht 72-78 kDa, 62 kDa
GenBank-ZugangsnummerNM_174936.3
Gene symbol PCSK9
Gene ID (NCBI) 255738
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial protein governing the circulating levels of low density lipoprotein-cholesterol (LDL-C), by virtue of its pivotal role in the degradation of the LDL receptor (LDLR). PCSK9 is expressed in the kidney and lung. It is synthesized as a 72 kDa immature precursor that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 63 kDa mature protein. The cleaved N-terminal fragment remains associated with the mature protein and is necessary for its secretion, allowing it to circulate in the blood. The ability of PCSK9 to regulate a diverse group of cell-surface proteins hinted that it might also be able to influence additional membrane proteins that are important in anti-tumour immune responses. Targeting PCSK9 to treat cancer is also attractive because two neutralizing antibodies against it, evolocumab and alirocumab, have already been approved for human clinical use to lower cholesterol levels. (PMID: 30522786, PMID: 22493497)

Protokolle

Produktspezifische Protokolle
WB protocol for PCSK9 antibody 27882-1-APProtokoll herunterladen
FC protocol for PCSK9 antibody 27882-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIHC

Biochimie

High-salt diet induces dyslipidemia through the SREBP2/PCSK9 pathway in dahl salt-sensitive rats

Authors - Ya-Nan Ou-Yang
mouseWB

bioRxiv

Effect of Total Sphingomyelin Synthase Activity on Low Density Lipoprotein Catabolism in Mice

Authors - Zhiqiang Li
human,mouseWB,IHC,IF

iScience

Targeting PCSK9 reduces cancer cell stemness and enhances antitumor immunity in head and neck cancer

Authors - Qi-Chao Yang
  • KD Validated
human,mouseWB

FASEB J

Exogenous hydrogen sulfide alleviates hepatic endoplasmic reticulum stress via SIRT1/FoxO1/PCSK9 pathway in NAFLD

Authors - Xiaomeng Cui
humanWB,IF

JACC Basic Transl Sci

PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia

Authors - Meixin Zhang
humanWB,IHC

Oncol Lett

PCSK9 promotes tumor cell proliferation and migration by facilitating CCL25 secretion in esophageal squamous cell carcinoma

Authors - Haijun Wang