PCDHA5 Polyklonaler Antikörper

PCDHA5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15270-1-AP

Synonyme

CNR6, CNRN6, CNRS6, CRNR6, PCDH alpha 5, PCDH ALPHA5, PCDHA5, protocadherin alpha 5

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 15270-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15270-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15270-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human gliomas using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Gliomgewebe, humanes Hirngewebe, humanes Lungengewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15270-1-AP bindet in WB, IHC, ELISA PCDHA5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCDHA5 fusion protein Ag7376
• Vollständiger Name: protocadherin alpha 5
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC033735
• Gene symbol: PCDHA5
• Gene ID (NCBI): 56143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.