PCDH10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PCDH10 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (2)

Kat-Nr. : 21859-1-AP

Synonyme

KIAA1400, OL PCDH, PCDH10, protocadherin 10

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 21859-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21859-1-AP (PCDH10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21859-1-AP (PCDH10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21859-1-AP (PCDH10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 21859-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21859-1-AP (PCDH10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

21859-1-AP bindet in WB, IHC, IF PCDH10 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCDH10 fusion protein Ag16494
• Vollständiger Name: protocadherin 10
• Berechnetes Molekulargewicht: 1040 aa, 113 kDa
• Beobachtetes Molekulargewicht: 113 kDa
• GenBank-Zugangsnummer: BC111560
• Gene symbol: PCDH10
• Gene ID (NCBI): 57575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Theranostics; A Compound AC1Q3QWB Selectively Disrupts HOTAIR-Mediated Recruitment of PRC2 and Enhances Cancer Therapy of DZNep.; Authors - Yansheng Li
human	WB	Clin Transl Med; Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR-576-5p/PCDH10 axis.; Authors - Jiahui Zhou
human	WB	Int J Biol Sci; FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes.; Authors - Xiao Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sampath (Verified Customer) (03-11-2020)

Pcdh10 antibody worked very well for me. I saw the bands even after 5 sec of exposure at dilution 1:1000 o/n incubation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

PCDH10 Antibody Western Blot, validation (1:1000 dilution) in Brain tissue (Cat no:21859-1-AP)

FH

Aurelie (Verified Customer) (06-13-2019)

In tissue, there is some background but the antibody give a correct signal. I also try in human primary fibroblasts, the background is lower and the band at 130kd observed here , is not present.- dilution 1/1500 should be enough

• Applications: Western Blot,
• Primary Antibody Dilution: 1/800
• Cell Tissue Type: Mouse Cortex

PCDH10 Antibody Western Blot, validation (1/800 dilution) in Mouse Cortex (Cat no:21859-1-AP)