PCBD1 Polyklonaler Antikörper

PCBD1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15702-1-AP

Synonyme

DCOH, Dimerization cofactor of HNF1, PCBD, PCBD1, PCD, PHS

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Galerie der Validierungsdaten

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  WB analysis of mouse pancreas using 15702-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15702-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15702-1-AP (PCBD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15702-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15702-1-AP (PCBD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15702-1-AP

  Immunofluorescent analysis of Hela cells, using PCBD1 antibody 15702-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15702-1-AP bindet in WB, IHC, IF,ELISA PCBD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCBD1 fusion protein Ag8331
• Vollständiger Name: pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
• Berechnetes Molekulargewicht: 104 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC006324
• Gene symbol: PCBD1
• Gene ID (NCBI): 5092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.