PAX8 Monoklonaler Antikörper

PAX8 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H7B3

Kat-Nr. : CL488-60145

Synonyme

paired box 8, Paired box protein Pax 8, PAX8

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Galerie der Validierungsdaten

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  IF Staining of SKOV-3 using CL488-60145

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 PAX8 antibody (CL488-60145, Clone: 4H7B3 ) at dilution of 1:800, CL594-Phalloidin (red).

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  IF Staining of human ovary tumor using CL488-60145

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 PAX8 antibody (CL488-60145, Clone: 4H7B3 ) at dilution of 1:100.

• 

  IF Staining of mouse kidney using CL488-60145

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite® Plus 488 PAX8 antibody (CL488-60145, Clone: 4H7B3 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: SKOV-3-Zellen, humanes Ovarialkarzinomgewebe, Mausnierengewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60145 bindet in IF PAX8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PAX8 fusion protein Ag0306
• Vollständiger Name: paired box 8
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC001060
• Gene symbol: PAX8
• Gene ID (NCBI): 7849
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).