PAWR Polyklonaler Antikörper

PAWR Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20688-1-AP

Synonyme

Par 4, PAR4, PAWR, Prostate apoptosis response 4 protein

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 20688-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20688-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

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  FC experiment of HeLa using 20688-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PAWR (20688-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

20688-1-AP bindet in WB, IHC, FC,ELISA PAWR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PRKC, apoptosis, WT1, regulator
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_002583
• Gene symbol: PAWR
• Gene ID (NCBI): 5074
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAWR, also named as PAR4, is a pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. It induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. PAWR inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. It down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. PAWR may be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. The antibody is specific to PAWR.

Publikationen

Species	Application	Title
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	IHC	Cancer Cell Int; Expression of PAWR predicts prognosis of ovarian cancer.; Authors - Jiahong Tan