Alpha Parvin/Actopaxin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Alpha Parvin/Actopaxin Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IF, CoIP,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 55268-1-AP

Synonyme

Actopaxin, Alpha parvin, CH ILKBP, MXRA2, PARVA, parvin, alpha, α-Parvin

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Galerie der Validierungsdaten

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  WB analysis of U2OS using 55268-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55268-1-AP

  WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

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  WB analysis of mouse kidney using 55268-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MDCK using 55268-1-AP

  Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, HeLa-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

55268-1-AP bindet in WB, IF, CoIP,ELISA Alpha Parvin/Actopaxin und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: parvin, alpha
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_018222
• Gene symbol: PARVA
• Gene ID (NCBI): 55742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.

Publikationen

Species	Application	Title
mouse	WB,CoIP	Proteome Sci; Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.; Authors - Lancia N F Darville