PARP2 Polyklonaler Antikörper

PARP2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 55149-1-AP

Synonyme

ADPRT 2, ADPRT2, ADPRTL2, ADPRTL3, hPARP 2, pADPRT 2, PARP 2, PARP2, poly (ADP ribose) polymerase 2, Poly [ADP ribose] polymerase 2, Poly[ADP ribose] synthase 2

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Galerie der Validierungsdaten

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  WB analysis using 55149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55149-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 55149-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55149-1-AP (PARP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, SH-SY5Y-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

55149-1-AP bindet in WB, IF, ELISA PARP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: poly (ADP-ribose) polymerase 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: NM_005484
• Gene symbol: PARP2
• Gene ID (NCBI): 10038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).

Publikationen

Species	Application	Title
human	WB	Oncogene; CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma.; Authors - Xueying Wang
	WB	Cells; Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development; Authors - Tatiana Kamaletdinova
human	WB	Eur J Med Chem; Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer.; Authors - Chunlan Pu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hasan (Verified Customer) (12-07-2022)

WB analysis for U2OS cells (WT and PARP2KO) with PARP2 Ab (55149-1-AP) at dilution of 1:2000 overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

PARP2 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:55149-1-AP)