PARL Polyklonaler Antikörper

PARL Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 26679-1-AP

Synonyme

MAMP, PACT, PARL, Pbeta, PRO2207, PSARL, PSARL1, PSENIP2, RHBDS1

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Galerie der Validierungsdaten

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  WB analysis of 3T3-L1 using 26679-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 26679-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26679-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, HEK-293-Zellen, HeLa-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

26679-1-AP bindet in WB, IHC, ELISA PARL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PARL fusion protein Ag24789
• Vollständiger Name: presenilin associated, rhomboid-like
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 36-42 kDa
• GenBank-Zugangsnummer: BC014058
• Gene symbol: PARL
• Gene ID (NCBI): 55486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARL (Presenilin associated rhomboid like), is a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. PARL has 2 isoforms produced by alternative splicing with the molecular mass of 42 kDa and 37 kDa. PARL regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of PARL may result in the release of a small peptide, P-beta, which may transit to the nucleus. The self-regulated cleavage of PARL at positions 52-53 (α-site) and 77--78 (β-site) produce two cleaved forms of PARL named MAMP and PACT with the molecular mass of 36 kDa and 33 kDa respectively (PMID: 14732705, 28178523).

Publikationen

Species	Application	Title
human		Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane
human,mouse	WB	Int J Mol Sci; The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.; Authors - Meiyu Cheng
human	WB	Cells; Disruption of Mitophagy Flux through the PARL-PINK1 Pathway by CHCHD10 Mutations or CHCHD10 Depletion; Authors - Tian Liu
human	WB	J Biol Chem; Diverse mitochondrial abnormalities in a new cellular model of TAFFAZZIN deficiency are remediated by cardiolipin-interacting small molecules.; Authors - Arianna F Anzmann
human	IHC	Front Pharmacol; Heat Shock Transcription Factor 2 Promotes Mitophagy of Intestinal Epithelial Cells Through PARL/PINK1/Parkin Pathway in Ulcerative Colitis.; Authors - Hao Liang
human,mouse	WB	Nat Cell Biol; Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space; Authors - Elias Adriaenssens