PARK7,DJ-1 Polyklonaler Antikörper

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• KD/KO Validated

PARK7,DJ-1 Polyklonal Antikörper für IHC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC

Konjugation

Biotin

Kat-Nr. : Biotin-11681

Synonyme

DJ 1, DJ1, FLJ27376, FLJ34360, FLJ92274, Oncogene DJ1, PARK7, Parkinson disease protein 7, Protein DJ 1

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Galerie der Validierungsdaten

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  IHC staining of mouse kidney using Biotin-11681

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using Biotin-11681 (PARK7,DJ-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using Biotin-11681

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using Biotin-11681 (PARK7,DJ-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-11681 bindet in IHC PARK7,DJ-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PARK7,DJ-1 fusion protein Ag2287
• Vollständiger Name: Parkinson disease (autosomal recessive, early onset) 7
• Berechnetes Molekulargewicht: 189 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa, 25 kDa
• GenBank-Zugangsnummer: BC008188
• Gene symbol: PARK7
• Gene ID (NCBI): 11315
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).