PARD3 Monoklonaler Antikörper

PARD3 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2B2

Kat-Nr. : 66968-1-Ig

Synonyme

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 66968-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66968-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66968-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66968-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66968-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 66968-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PARD3 antibody (66968-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of MCF-7 using 66968-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PARD3 (66968-1-Ig, Clone:1D2B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66968-1-Ig bindet in WB, IF, FC, ELISA PARD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PARD3 fusion protein Ag7656
• Vollständiger Name: par-3 partitioning defective 3 homolog (C. elegans)
• Berechnetes Molekulargewicht: 151 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC011711
• Gene symbol: PARD3
• Gene ID (NCBI): 56288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.