PAPD5 Polyklonaler Antikörper

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• KD/KO Validated

PAPD5 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 55197-1-AP

Synonyme

PAPD5, TENT4B, Terminal uridylyltransferase 3, TRF4 2, TUTase 3

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Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 55197-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55197-1-AP (PAPD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 55197-1-AP

  IP Result of anti-PAPD5 (IP:55197-1-AP, 3ug; Detection:55197-1-AP 1:500) with Jurkat cells lysate 1600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

55197-1-AP bindet in WB, IP, ELISA PAPD5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PAP associated domain containing 5
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 63-80 kDa
• GenBank-Zugangsnummer: NM_001040285
• Gene symbol: PAPD5
• Gene ID (NCBI): 64282
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAPD5, also named as Non-canonical poly(A) RNA polymerase PAPD5, is a 572 amino acid protein, which contains 1 PAP-associated domain and belongs to the DNA polymerase type-B-like family. PAPD5 is predominantly expressed in the cytoplasm. PAPD5 as catalytic subunit of a TRAMP-like complex that has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. PAPD5 plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. PAPD5 also may play a role in sister chromatid cohesion. The calculated molecular weight of PAPD5 is a 63 kDa, but the post-modified protein is about 70-80 kDa. PAPD5 exists some isoform with the molecular weight about 50 kDa and 70-80 kDa.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan ; KD Validated